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Biblioteca (s) : |
INIA Las Brujas. |
Fecha : |
09/11/2017 |
Actualizado : |
12/12/2018 |
Tipo de producción científica : |
Artículos en Revistas Indexadas Internacionales |
Autor : |
FEDERICI, M.; BAJSA, N.; LAGURARA, P.; REVALE, S.; MARCONDES, J.A.; DALLA RIZZA, M. |
Afiliación : |
MARIA TERESA FEDERICI RODRIGUEZ, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; NATALIA BAJSA VALVERDE, MEC/ IIBCE (Instituto de Investigaciones de Ciencias Biológicas "Clemente Estable"); PAULA LAGURARA, MEC/ IIBCE (Instituto de Investigaciones de Ciencias Biológicas "Clemente Estable"); SANTIAGO REVALE, INDEAR (Instituto de Agrobiotecnología de Rosario); JACKSON A. MARCONDES, Universidad Estadual Paulista (UNESP)/ Campus de Jaboticabal; MARCO DALLA RIZZA VILARO, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay. |
Título : |
Soil and rhizosphere bacterial diversity in maize agro-ecosystem. |
Fecha de publicación : |
2017 |
Fuente / Imprenta : |
Sustainable Agriculture Research, 2017, v.6 (3): 35-51. |
ISSN : |
1927-050X (Print) // 1927-0518 (Online) |
DOI : |
10.5539/sar.v6n3p |
Idioma : |
Inglés |
Notas : |
Article history: Received: April 3, 2017; Accepted: May 20, 2017; Online Published: June 16, 2017. |
Contenido : |
ABSTRACT.
Management practices used in maize production have an impact on soil agro- ecosystems where different microbial communities coexist. Soil inhabiting bacteria are numerous and diverse, but we know very little about their ecological distribution. Here we analyzed the bacterial community diversity in the rhizosphere of two transgenic maize cultivars, in agricultural soil before sowing and in non-cultivated soil in an experimental site in the south region of Uruguay. We followed two culture-independent methods: DGGE (denaturing gradient gel electrophoresis) and 454-pyrosequencing of 16S rRNA gene amplicon. Through pyrosequencing, the three environments analyzed presented differences in terms of bacterial composition. However, no differences were found in the relative abundance of the ten most represented phyla in the rhizosphere of the two cultivars at different phenological stages. We found significant differences of Bacteroidetes, Gemmatimonadetes, Planctomycetes, Proteobacteria and Verrucomicrobia phyla when comparing agricultural and non-cultivated soils, as well as a significant enrichment of members of the phylum Gemmatimonadetes in all rhizosphere samples compared to soil. Through DGGE analysis we evidenced that maize rhizosphere bacterial communities changed at different phenological stages in both cultivars. We also provided baseline information about bacterial specific taxa within maize agro- ecosystem for further evaluation of possible rhizosphere bacterial community shifts of genetically modified maize cultivars under different management practices.
Copyright © Canadian Center of Science and Education MenosABSTRACT.
Management practices used in maize production have an impact on soil agro- ecosystems where different microbial communities coexist. Soil inhabiting bacteria are numerous and diverse, but we know very little about their ecological distribution. Here we analyzed the bacterial community diversity in the rhizosphere of two transgenic maize cultivars, in agricultural soil before sowing and in non-cultivated soil in an experimental site in the south region of Uruguay. We followed two culture-independent methods: DGGE (denaturing gradient gel electrophoresis) and 454-pyrosequencing of 16S rRNA gene amplicon. Through pyrosequencing, the three environments analyzed presented differences in terms of bacterial composition. However, no differences were found in the relative abundance of the ten most represented phyla in the rhizosphere of the two cultivars at different phenological stages. We found significant differences of Bacteroidetes, Gemmatimonadetes, Planctomycetes, Proteobacteria and Verrucomicrobia phyla when comparing agricultural and non-cultivated soils, as well as a significant enrichment of members of the phylum Gemmatimonadetes in all rhizosphere samples compared to soil. Through DGGE analysis we evidenced that maize rhizosphere bacterial communities changed at different phenological stages in both cultivars. We also provided baseline information about bacterial specific taxa within maize agro- ecosystem for further evaluation of possible rhizosphere bacterial ... Presentar Todo |
Palabras claves : |
DGGE; METAGENOMICS; PYROSEQUENCING; RHIZOSPHERE; SOIL BACTERIA; TRANSGENIC MAIZE. |
Asunto categoría : |
-- |
URL : |
http://www.ainfo.inia.uy/digital/bitstream/item/7925/1/67345-252782-1-PB.pdf
http://www.ccsenet.org/journal/index.php/sar/article/view/67345/37668
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Marc : |
LEADER 02541naa a2200289 a 4500 001 1057747 005 2018-12-12 008 2017 bl uuuu u00u1 u #d 022 $a1927-050X (Print) // 1927-0518 (Online) 024 7 $a10.5539/sar.v6n3p$2DOI 100 1 $aFEDERICI, M. 245 $aSoil and rhizosphere bacterial diversity in maize agro-ecosystem.$h[electronic resource] 260 $c2017 500 $aArticle history: Received: April 3, 2017; Accepted: May 20, 2017; Online Published: June 16, 2017. 520 $aABSTRACT. Management practices used in maize production have an impact on soil agro- ecosystems where different microbial communities coexist. Soil inhabiting bacteria are numerous and diverse, but we know very little about their ecological distribution. Here we analyzed the bacterial community diversity in the rhizosphere of two transgenic maize cultivars, in agricultural soil before sowing and in non-cultivated soil in an experimental site in the south region of Uruguay. We followed two culture-independent methods: DGGE (denaturing gradient gel electrophoresis) and 454-pyrosequencing of 16S rRNA gene amplicon. Through pyrosequencing, the three environments analyzed presented differences in terms of bacterial composition. However, no differences were found in the relative abundance of the ten most represented phyla in the rhizosphere of the two cultivars at different phenological stages. We found significant differences of Bacteroidetes, Gemmatimonadetes, Planctomycetes, Proteobacteria and Verrucomicrobia phyla when comparing agricultural and non-cultivated soils, as well as a significant enrichment of members of the phylum Gemmatimonadetes in all rhizosphere samples compared to soil. Through DGGE analysis we evidenced that maize rhizosphere bacterial communities changed at different phenological stages in both cultivars. We also provided baseline information about bacterial specific taxa within maize agro- ecosystem for further evaluation of possible rhizosphere bacterial community shifts of genetically modified maize cultivars under different management practices. Copyright © Canadian Center of Science and Education 653 $aDGGE 653 $aMETAGENOMICS 653 $aPYROSEQUENCING 653 $aRHIZOSPHERE 653 $aSOIL BACTERIA 653 $aTRANSGENIC MAIZE 700 1 $aBAJSA, N. 700 1 $aLAGURARA, P. 700 1 $aREVALE, S. 700 1 $aMARCONDES, J.A. 700 1 $aDALLA RIZZA, M. 773 $tSustainable Agriculture Research, 2017$gv.6 (3): 35-51.
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Registro original : |
INIA Las Brujas (LB) |
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Registro completo
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Biblioteca (s) : |
INIA La Estanzuela. |
Fecha actual : |
09/10/2014 |
Actualizado : |
30/07/2019 |
Tipo de producción científica : |
Poster |
Autor : |
PÉREZ BIDEGAIN, M.; SAWCHIK, J.; BARRETO, P.; PÉREZ, M.M. |
Afiliación : |
JORGE SAWCHIK PINTOS, Instituto Nacional de Investigación Agropecuaria (INIA), Uruguay. |
Título : |
Pesticide runoff into surface water in a mollisol under no tillage. |
Fecha de publicación : |
2012 |
Fuente / Imprenta : |
In: INTERNATIONAL SOIL TILLAGE RESEARCH ORGANIZATION. 19., SOCIEDAD URUGUAYA DE CIENCIA DEL SUELO, 4., 2012, Montevideo, UY. Poster presentation: 372. Montevideo, UY: ISTRO, 2012. |
Idioma : |
Inglés |
Palabras claves : |
ÁCIDO AMINOMETILFOSFÓRICO; AMPA; MOLISOLES. |
Thesagro : |
AGRICULTURA CONTINUA; CERO LABOREO; CLORPIRIFOS; ESCURRIMIENTO; GLIFOSATO; GLYCINE MAX; LLUVIA; SOJA. |
Asunto categoría : |
P01 Conservación de la naturaleza y recursos de La tierra |
Marc : |
LEADER 00858nam a2200265 a 4500 001 1051002 005 2019-07-30 008 2012 bl uuuu u00u1 u #d 100 1 $aPÉREZ BIDEGAIN, M. 245 $aPesticide runoff into surface water in a mollisol under no tillage.$h[electronic resource] 260 $aIn: INTERNATIONAL SOIL TILLAGE RESEARCH ORGANIZATION. 19., SOCIEDAD URUGUAYA DE CIENCIA DEL SUELO, 4., 2012, Montevideo, UY. Poster presentation: 372. Montevideo, UY: ISTRO$c2012 650 $aAGRICULTURA CONTINUA 650 $aCERO LABOREO 650 $aCLORPIRIFOS 650 $aESCURRIMIENTO 650 $aGLIFOSATO 650 $aGLYCINE MAX 650 $aLLUVIA 650 $aSOJA 653 $aÁCIDO AMINOMETILFOSFÓRICO 653 $aAMPA 653 $aMOLISOLES 700 1 $aSAWCHIK, J. 700 1 $aBARRETO, P. 700 1 $aPÉREZ, M.M.
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